KCOM medium 2 ;

Actinobacillus actinomycetemcomitans medium

(Dentaid-1; JCM. Feb. 2001, p. 509–513)

1. Agar plate

Brain Heart Infusion broth 37.0 g (3.7%)

Yeast extract 5.0 g (0.5%)

Sodium fumarate (Sigma) 1.5 g (0.15%)

Sodium formate (Sigma) 1.0 g (0.1%)

Bacto-agar 20.0 g (2.0%)

DW 1000 ㎖

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▶ Autoclave

▶ After autoclave, maintain at 45-50℃

▶ Add ;

Vancomycin (5㎎/㎖) 1.8 ㎖ (9㎍/㎖)

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▶ Store at 4℃

2. Cultrue conditions

▶ Temperature : 37 ℃

▶ Atmosphere : 5% CO2 incubator

3. Broth

agar만 넣지 않고 동일한 과정으로 제조