【 KCOM medium 2 】 

    - A. actinomycetemcomitans selective agar (TSBV)

Tryptic soy agar 40.0 gm

Yeast extract 1.0 gm

DW upto 898.0 ml

…………………………………………………………………………………………………………………………… 

→ Autoclaving and cool down to 45°C

Horse serum 100 ml (final conc. 10%)

Bacitracin stock (75 mg/ml) 1 ml (final conc. 75 μg/ml)

Vancomycin stock (5 mg/ml) 1 ml (final conc. 5 μg/ml)

* Bacitracin solution can be used for 1 week after making.